Enhanced Detection of Enteroviruses in Clinical Samples by Reverse Transcription-PCR Using Complementary Locked Primer Technology
نویسندگان
چکیده
منابع مشابه
Enhanced detection of enteroviruses in clinical samples by reverse transcription-PCR using complementary locked primer technology.
To increase detection sensitivity, we modified primers using complementary locked primer (CLP) technology. The sensitivity of the reverse transcription-PCR (RT-PCR) with CLP-modified primers was 10- to 100-fold higher than that of RT-PCR without these primers. CLP-modified primers can increase sensitivity, providing a widely accessible method for molecular diagnosis.
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 2010
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.01790-09